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論文投稿 Effect of lower chlorinated hydroxylated-polychlorobiphenyls on development of PC12 cells

作成年度 2017 年度
論文名 Effect of lower chlorinated hydroxylated-polychlorobiphenyls on development of PC12 cells
論文名(和訳)
論文副題
発表会
誌名 Environ Sci Pollut Res
巻・号・回 7 
発表年月日 2017/07/01
所属研究室/機関名 著者名(英名)
Water quality team, Water Environment Research groupSatomi Mizukami-Murata
AISTKatsuhide Fujita
Osaka Univ.Takeshi Nakano
抄録
Hydroxylated polychlorobiphenyls (OH-PCBs) are major metabolites of PCBs that are widely distributed in the environment. While the effects of penta- to heptachlorinated OH-PCBs on neuronal differentiation have been widely reported, those of lower chlorinated OHPCBs have not been extensively studied. To investigate the effects of lower chlorinated OH-PCBs on neuronal development, we studied the effects of mono- to hexachlorinated OH-PCBs on PC12 cells. Morphological changes were examined using an automatic system IN Cell Analyzer. Seventeen of the 20 OH-PCBs investigated promoted neuronal elongation in an OH-PCB concentration- dependent manner, while three OH-PCB congeners suppressed neuronal elongation based on Dunnett’s analysis. In particular, the top five OH-PCBs (4OH-PCB2, 4′ OH-PCB3, 4′OH-PCB25, 4′OH-PCB68, and 4′OHPCB159), which have hydroxyl groups at the para-position and chlorine substitutions at the 2, 4, or 3′ positions, significantly promoted neuronal elongation. Moreover, these neuronal elongations were suppressed by U0126, and phosphorylation of extracellular signal-regulated kinase (ERK) 1/2 was observed in PC12 cells treated with 4OH-PCB2, 4′OH-PCB25, and 4′OH-PCB159. Taken together, our results indicate that the effect of OH-PCB on neuronal development is not dependent on the number of chlorine groups but on the chemical structure, and the mitogen-activated kinase kinase (MEK)-ERK1/2 signaling pathway is involved in this process.
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